Correction: G-Protein-Coupled Estrogen Receptor Agonist Suppresses Airway Inflammation in a Mouse Model of Asthma through IL-10
نویسندگان
چکیده
In the Materials and Methods section, there is an error in the fifth and sixth sentences of the section titled “Flow cytometry.” This sentence should read: 2), PE-Cy5-conjugated anti-TCRβ (BioLegend, San Diego, CA), PE-Cy7-conjugated ant-NK1.1 (BioLegend), PE-Cy7-conjugated anti-CD8a (BioLegend), PE-CF594-conjugated anti-CD4 (BD Biosciences), and GPR30 (N15)-R Antibody (Santa Cruz Biotechnology, Santa Cruz, CA) with subsequent staining by Alexa Fluor 488-conjugated goat anti-rabbit immunoglobulin G (IgG) (Invitrogen, Grand Island, NY). Then, the cells were washed, permeabilized with BD Cytofix/Cytoperm (BD Biosciences), and stained with PE-conjugated anti-IL-10 (BioLegend) to detect intracellular IL-10.
منابع مشابه
G-Protein-Coupled Estrogen Receptor Agonist Suppresses Airway Inflammation in a Mouse Model of Asthma through IL-10
Estrogen influences the disease severity and sexual dimorphism in asthma, which is caused by complex mechanisms. Besides classical nuclear estrogen receptors (ERαβ), G-protein-coupled estrogen receptor (GPER) was recently established as an estrogen receptor on the cell membrane. Although GPER is associated with immunoregulatory functions of estrogen, the pathophysiological role of GPER in aller...
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